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KMID : 1140120120170040364
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Cancer Prevention Research
2012 Volume.17 No. 4 p.364 ~ p.371
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Induction of Apoptosis and Telomerase Activity of NCI-H460 Human Lung Carcinoma Cells by Methanol Extract of Houttuynia cordata Thunberg
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Choi Yung-Hyun
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Abstract
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Houttuynia cordata Thunb. is a medicinal plant of the Saururaceae family widely used in folk medicine in several Asian countries. It has been reported that extracts of H. cordata exhibit anti-cancer activity through apoptosis induction, although the mechanisms are not fully understood yet. In the present study, it was investigated the further possible mechanisms of anti-proliferative effects by the methanol extract of H. cordata (MEHC) in cultured human lung carcinoma NCI-H460 cells. The results indicated that exposure of NCI-H460 cells to MEHC resulted in cell cycle arrest at G2/M phase and induction of apoptosis, which was associated with inhibition of the expression of cyclin B1 and activation of caspases, respectively. Moreover, MEHC inhibited telomerase activity through down-regulation of the expression of human telomerase reverse transcritase and c-myc. Taken together, together with previous findings, the data provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.
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KEYWORD
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Houttuynia cordata Thunb., NCI-H460 cells, G2/M arrest, Apoptosis, Telomerase
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